Figure 6: Exosomes released from DCs infected with HIV-1 increase apoptosis of CD4TL. DCs were cultured in the presence of NL4-3Balenv for 48h. Exosomes in the cell-free supernatant were isolated from virions by immunocapture using protein A/G beads pre-coated with anti-AChE (AE-1) or with an isotype (IgG1) antibody (control). The suspension eluted from the beads (AE-1) was added to a culture of resting autologous CD4TL. FITC-VADFMK was added 30 min before flow cytometry analysis. Data are expressed as a percentage of positive cells for FITC-VAD-FMK and represent the mean ± SEM from four independent experiments.