Figure 2: Results of histopathological, DIF, and IB studies. (a,b) Histopathological features (hematoxylin–eosin stain) (a: ×100; b: ×400). (c) The results of DIF for C3 (×100). (d) IB of normal human dermal extracts. EBA control serum reacted with the 290-kDa type VII collagen (lane 1), and anti-laminin gamma-1 pemphigoid control serum reacted with the 200-kDa laminin gamma-1 (lane 2). Lanes 3 and 4 reveal the IgG and IgA antibodies in the patient, respectively. (e) IB of purified human laminin-332. The control serum of anti-laminin-332-type MMP reacted with the 165-kDa and 145-kDa alpha-3, 140-kDa beta-3, and the 105-kDa gamma-2 subunits (lane 1), but the normal control serum showed no positive reactivity (lane 2). Lanes 3 and 4 are for IgG and IgA antibodies in the patient, respectively, and molecular weights are indicated in the left (d and e).