Individuals Date of collection Laboratory results ELISA IIF PCR 1 20-Jun-08 neg neg np 3-Mar-09 neg neg neg 7-Aug-09 neg neg neg 23-Jul-10 neg neg neg 5-May-11 np np np 2 20-Jun-08 neg neg np 3-Mar-09 neg neg neg 7-Aug-09 neg neg neg 23-Jul-10 neg neg pos 5-May-11 neg neg neg 3 20-Jun-08 neg 20-Jan np 3-Mar-09 neg 20-Jan neg 7-Aug-09 neg neg neg 23-Jul-10 neg 20-Jan neg 5-May-11 neg neg neg 4 20-Jun-08 neg neg neg 3-Mar-09 neg neg neg 7-Aug-09 neg neg neg 23-Jul-10 np np np 5-May-11 np np np 5 20-Jun-08 neg neg neg 3-Mar-09 neg neg neg 7-Aug-09 neg neg neg 23-Jul-10 neg neg pos 5-May-11 (0,800) Jan-40 neg 6 20-Jun-08 neg neg neg 3-Mar-09 neg neg neg 7-Aug-09 np np np 23-Jul-10 np np np 5-May-11 np np np

Table 1: Laboratory results, on different sampling dates, for samples from individuals with subclinical infections, resident in the state of Paraná, southern Brazil. Pos=positive; neg=negative; np=not performed.