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Figure 1: a) H&E. Low magnification (10X) demonstrates edema and an inflammatory infiltrate in the superficial dermis, and subepidermal clefting at the basement membrane zone (black arrows). b) H&E highlighting spongiosis in the epidermis (black arrow) and the lymphohisticcitic infiltrate in the papillary dermis (red arrow) (40X). c) Demonstrates the patient’s clinical lesions. d) DIF documenting a positive pseudo-lupus band at the BMZ (red arrow), visualized via FITC conjugated anti-human fibrinogen (green staining; red and yellow arrows). e) Dual DIF staining, highlighting overexpression of Cy5 conjugated JAM-A (pink) in the same areas where the pseudo-lupus fibrinogen band is present Note the JAM-1 staining overlaps with the FITC conjugated antihuman fibrinogen staining (yellow/green staining) (blue arrow). f) Higher magnification of the DIF pseudo-lupus band (red arrow). |