Figure 3: Immunostaining for RSAD2 with Vector Blue (blue) and counterstained with neutral red (pink). (B) Representative sections from eyes with drusen ≥ 25 μm shown that areas around drusen (≥ 25 μm), in RPE and Bruch’s membrane are more immunoreactive for RSAD2than (A) the background reactivity seen in tissues with small (≤ 25 μm) or no drusen. Scale bar 25 μm. (C) Eyes with clinically significant drusen ( ≥ 25 μm, n = 15) are found to have stronger immunostaining for RSAD2 particularly in the Bruch’s membrane and choroid (p ≤ 0.05, black asterisk) than those without clinically significant drusen (n=13).