Figure 9: RGC axonal transport and GFAP expression predict gp130 expression in the ganglion cell and nerve fiber layers of aging retina. Left panels: Regression graphs of fluorescent intensity for gp130 immunolabeling and intensity of cholera toxin-β (CTB) uptake/transport (A) and GFAP immunolabeling (B) in the ganglion cell and nerve fiber layers of mid-central to mid-peripheral regions of whole mounted retina from aged C57 mice. Data is plotted as intensity of gp130 (y-axis) versus intensity of CTB (x-axis; A) and GFAP (x-axis; B) in arbitrary units. Solid line indicates regression line based on r2 value. Dashed line indicates the 95% confidence interval. Dotted line indicates predicted values based on r2 and 95% confidence. Right panels: Representative confocal micrographs depicting relationships between gp130 immunolabeling (green) and CTB transport (red; A) and GFAP immunolabeling (blue; B). For all, examples of low (left) and high (right) intensities are provided. Scaling is consistent for all images.