Figure 1: Diabetic wild type (gHb= 11.0 ± 1.8) and IL-1R1M-/- (gHb= 12.1 ± 0.4) were sacrificed after 7 months of diabetes along with age matched normal controls (wild type gHb= 3.8 ± 0.55, IL-1R1-/- gHb= 3.2 ± 0.2). Animals were scarified and eyes were isolated and fixed in formalin. Retinal sections were processed for both glutamine synthase, CRALBP, and DAPI staining and blinded samples were visualized using confocal microscopy Z-sections. The number of Müller cells was determined by counting three independent areas per retinal section and three retinal sections per animal. The number of Müller cells per mm2 retina is expressed as mean ± STDV (n=10 per group).