OCM1   C918   Total E+05 Viability %   Total E+05 Viability % Run 1     Run 1     Control 1.98 75.74 Control 1.74 76.92 ASC1* 2.18 71.12 ASC1 2.64 80.32 ASC3 1.92 38.95 ASC3 1.68 61.06 ASC5 1.93 20.81 ASC5 1.62 41.97 ASC7 1.91 3.66 ASC7 2.32 11.91 ATO2 2.28 61.10 ATO2 2.27 60.17 ATO4 2.03 54.10 ATO4 2.70 54.10 ATO6 2.23 52.70 ATO6 3.61 53.00 ATO8 2.25 46.00 ATO8 3.96 55.70 Run 2     Run 2     Control 2.07 77.51 Control 1.85 93.19 ASC1 2.17 67.74 ASC1 1.66 39.02 ASC3 2.24 12.86 ASC3 1.85 9.50 ASC5 2.41 4.76 ASC5 2.29 2.56 ASC7 2.11 2.78 ASC7 1.91 2.49 ATO2 2.01 61.00 ATO2 1.59 71.05 ATO4 2.62 65.46 ATO4 1.71 61.80 ATO6 2.70 64.01 ATO6 2.13 61.18 ATO8 3.18 65.50 ATO8 1.96 64.39 Run 3     Run 3     Control 1.78 91.97 Control 1.48 89.62 ASC1 1.44 92.04 ASC1 1.40 93.59 ASC3 1.30 70.17 ASC3 .995 21.35 ASC5 1.15 38.93 ASC5 1.47 4.19 ASC7 1.35 18.84 ASC7 1.53 2.37 ATO2 1.45 69.69 ATO2 1.13 73.27 ATO4 1.51 61.11 ATO4 1.51 57.04 ATO6 1.58 62.06 ATO6 1.56 56.91 ATO8 1.63 65.01 ATO8 1.60 53.77

Table 1: Cell viability of OCM1 and C918 UM cell lines after exposure to increasing doses of both ASC (sodium ascorbate) and ATO (arsenic trioxide). ASC 1, 3, 5, and 7 correspond to concentrations of 1, 3, 5, and 7 mM/ml of ASC. ATO 2, 4, 6, and 8, correspond to doses of 2, 4, 6, and 8 μg/ml of ATO. Run 1, 2, and 3=experiment no. 1, 2, and 3. TOT (E+05)=total number of cells × 100,000. Viability%=percentage of live cells. Control=untreated samples.