Figure 4: Protein levels and methylation status of ERCC6 in HLE B-3 after treatment with 5-aza-dC. (A) Protein expression of ERCC6 in control cells and in cells after treatment with 5-aza-dC. Relative ERCC6 protein level to GAPDH is presented as mean ± SD. *: p<0.01 (B) Bisulfite genomic sequencing of the ERCC6 CGrich sequence of the CpG island in control cells and in cells after treatment with 5-aza-dC. (C) After treatment with 5-aza-dC, the cells displayed hypomethylation compared to the control cells. * P<0.01 vs. control.