Figure 3: Schematic of the PCO model. A cell culture insert is placed into a well of a 12 well plate. The ground of the insert consists of a PET membrane with 1 μm pores allowing flow between the upper and lower chamber. The used PET membrane was coated with type IV collagen and one of the three IOLs was then placed on it and pushed down with a small weight. The upper chamber contained LECs. LECs were cultured for six days before the membrane was removed and stained for determination of cell proliferation and migration.