Figure 1: PEDF is constitutively expressed in healthy retina. A. Graphical representation of pedf total mRNA levels in naïve C57 retina as measured by qRT-PCR. Y-axis represents the average delta of threshold cycle (Δ-CT) values for pedf normalized to the control gene gapdh. B. Representative micrograph of longitudinal sections of retina from naïve mice immunolabeled with antibody against PEDF with (right panel) and without (left) pre-absorption with recombinant PEDF (rPEDF). Morphology consistent with localization to RGCs (white arrowheads) and Müller cells (black arrowheads). C. Retinal layer-specific quantification of PEDF labeling, expressed in intensity (arbitrary units) per area (mm2). All asterisks denote p<0.05. ONL: Outer Nuclear Layer; OPL: Outer Plexiform Layer; INL: Inner Nuclear Layer; IPL: Inner Plexiform Layer; GCL: Ganglion Cell Layer; NFL: Nerve Fiber Layer.