Figure 6: Elevated IOP increases PEDF-R expression in murine retina. A. Graphical representation of pedf-r total mRNA in retina from saline- and microbead-injected mice, as measured by qPCR. y-axis represents fold change in Δ-CT values for pedf-r (normalized to the control gene gapdh) for microbead-injected versus saline-injected. Asterisk denotes p<0.05. B. Representative micrographs of immunolabeling for PEDF-R in longitudinal sections of retina from saline- (right panel) and microbead-injected (left panel) mice demonstrate increased intensity of labeling, particularly in the IPL, GCL and NFL, as compared to saline-injected eyes. The pattern of PEDF-R immunolabeling is consistent with RGC soma in the GCL (white arrowheads) and Müller cell processes and endfeet in the ONL and NFL (black arrowheads), respectively. C. Retinal layerspecific quantification of PEDF-R labeling, expressed in intensity (arbitrary units) per area (mm2). All asterisks denote p<0.05. ONL: Outer Nuclear Layer; OPL: Outer Plexiform Layer; INL: Inner Nuclear Layer; IPL: Inner Plexiform Layer; GCL: Ganglion Cell Layer; NFL: Nerve Fiber Layer