Figure 6: Restriction fragment length polymorphism analysis. 6a)Wild type versus mutant sequence with restriction site for theenzyme HpyCH4III WT-Wild type; M- Mutant. 6b) Restrictiondigestion of exon #6 with HpyCH4III of hBEST1 of family SEB1.The Proband (P) (III.1), Proband’s Mother (PM) (II.4) andProband’s Sib (PS) (III.2) have 481bp, 442 bp. 220 bp & 39bpfragments which results from the digestion of the mutant allele,whereas the Proband’s father (PF) (II.5) and Control (C) have 481 &220bp fragments that depicts the wild type allele. The lane Mindicates DNA ladder (100 bp – 1000 bp), UD denotes UndigestedPCR product.