Figure 1: Retinal level of BDNF and TrkB at different ages in DBA/2J mice. A. IOP recorded by TonoLab was plotted as mean value (mmHg) in C57BL/6J and 3-4 month (3), 7 month, 10-12 month (10) and 15-18 month (18) DBA/2J mice. B. Protein extracts were resolved by SDS-PAGE and immunoblotted with antibodies against BDNF (14 kDa), total-TrkB (140 kDa) and β-tubulin (55 kDa). Neurotrophin and its receptor modulation was evaluated normalizing BDNF and TrkB levels on loading control, β-tubulin. Data are reported as mean ± SEM (Standard Error of the Mean) and expressed as percent of control (3 month DBA/2J mice; at least three retinas/group were processed); *p<0.05.