S.No No. of colonies at 35 KHz Temp OC No. of colonies at130KHz Temp OC Time, Min 1. 1086 31 1298 32 5 2. 827 32 756 35 10 3. 742 34 492  37 20 4. 556 37 373 40 30 5. Control-1525 30   30

Table 2: Average colonies obtained on LB plates.