Results were normalized to 18S rRNA measured in parallel reactions. Graphs depict relative levels of gene expression for (A) neuronal Hu, (B) myelinassociated glycoprotein-1 (MAG-1), (C) glial fibrillary acidic protein (GFAP), (D) allograft inflammatory factor -1 (AIF), (E) acetylcholinesterase (AChE), and (F) choline acetyltransferase (ChAT), and. Inter-group comparisons were made using Student t-tests. Significant P-values are shown over the graphs.

Figure 3: Early postnatal binge ethanol exposures alter neuronal and glial gene expressions in early adolescent cerebella. RNA extracted from cerebella (N=8 samples per group) was reverse transcribed, and the cDNAs were used to measure gene expression by qPCR analysis.