Cerebella protein homogenates were used to measure immunoreactivity to
(A) insulin receptor (IR), (D) IGF-1R, (G) IRS-1, (B) pYpY1162/1163-IR, (E)
pYpY1135/1136-IGF-1R, (H) pS312-IRS-1 using the bead-based Akt and
phospho-specific Akt pathway multiplex ELISA kits. Phospho-/total protein
ratios for (C) IR, (F) IGF-1R, and (I) IRS-1 were calculated. Comparisons (N=8
samples per group) were made using Student T-tests. Significant differences
are indicated within the panels.