| Test |
Time Point |
| A. Metaphase cytogenetics
B. FISH (peripheral blood sample is acceptable if bone marrow specimen not available) using dual color, dual fusion probes and standard technique
|
1. At diagnosis and 6, 12, and 18 months until CCyR achieved. If CCyR achieved at an earlier time point, then there is no need to perform metaphase cytogenetics in a stable patients.
2. Rising level of BCR-ABL1 transcript (1 log increase) without achieving MMR |
| QRT-PCR for BCR-ABL transcript |
1. At diagnosis to establish baseline transcript level.
2. Every 3 months thereafter until patient is responding to TK inhibitors, and every 3-6 months after achieving CCyR
3. If level of BCR-ABL1 transcript is rising (1 log increase) after achieving MMR, then QRT-PCR should be performed every 1-3 months |
| Kinase domain mutation testing |
1. At time of suboptimal response or failure
2. Before switching to another TK inhibitor
|
