Figure 1: Histologic effects of PPAR agonists on alcohol-induced steatohepatitis. Adult male Long Evans rats were chronically fed with liquid diets containing (A-D) 0% (control) or (EH) 37% ethanol, and treated by i.p. injection of (A,E) vehicle (saline), or a (B,F) PPAR-α (GW7647; 25 μg/Kg), (C,G) PPAR-δ (L-160,043; 2 μg/Kg), or (D,H) PPAR-γ (F-L-Leu; 20 μg/Kg) agonist (See Materials and Methods). Formalin-fixed paraffin-embedded sections (5 μm thick) of liver were stained with H&E. Zone 3 hepatocytes are depicted at or near the center of each image. Note regular chord-like architecture, uniform hepatocyte morphology and absence of steatosis in control livers, the loss of chord-like architecture, steatosis (arrow) and inflammatory infiltrate (circle) in the ethanol-exposed, vehicle- and PPAR-α agonist treated livers, and restoration of hepatic architecture in ethanol+ PPAR-δ and PPAR-γ treated livers. Original magnifications, 200x.