Figure 2: Histologic responses to PPAR agonist treatments of experimental chronic alcohol-induced steatohepatitis. Adult male Long Evans rats were fed with diets containing (AD) 0% or (E-H) 37% ethanol and treated with (A,E) vehicle, or a (B,F) PPAR-α, (C,G) PPAR-δ, or (D,H) PPAR-γ agonist. Formalinfixed paraffin-embedded sections (5 μm thick) of liver were stained with H&E. Zone 3 hepatocytes are depicted. Note the uniform hepatocyte cytology in control livers versus the prominent steatosis (clear cytoplasmic vacuoles, e.g. upper right inset in E), inflammation (lower insets in E and H) and apoptosis (lower inset in E) in the ethanolexposed, vehicle-, PPAR-α, or PPAR-γ treated livers. Original magnifications, 400x.