Figure 3: Effects of PPAR agonists on ethanol-induced hepatic steatosis. Formalinfixed, cryostat sections (10 μm thick) of liver from (A-D) control or (E-H) ethanol-fed rats treated with (A,E) vehicle, or a (B,F) PPAR-α, (C,G) PPAR-δ, or (D,H) PPAR-γ agonist were stained with Oil Red O to detect cytoplasmic lipid accumulation (red punctate labeling). Note strikingly lower levels of Oil Red O staining in (A-D) control and (H) ethanol+PPAR-δ agonist treated livers compared with (E,F,H) ethanol-fed and vehicle, PPAR-α, or PPAR-γ agonist treated livers. Original magnifications, 400x.