Figure 4: Effects of PPAR agonists on ethanol-induced hepatic fibrosis. Formalin-fixed, paraffin-embedded sections (5 μm thick) of liver from (A-D) control or (E-H) ethanol-fed rats that were treated with (A,E) vehicle, or a (B,F) PPAR-α, (C,G) PPAR-δ, or (D,H) PPAR-γ agonist were stained with Sirius red to detect collagen (wavy red linear labeling). Note minimal fibrosis in (A-D) control and (H) ethanol+PPAR-γ agonist treated livers compared with the (EG) delicate peri-hepatocyte and sinusoidal labeling of collagen fibrils in ethanolexposed vehicle, PPAR-α, or PPAR-δ agonist treated livers. Original magnifications, 400x.