| Treatment |
Control |
Ethanol |
| Vehicle |
Absent |
Extensive |
| PPAR-α |
Absent |
Moderate |
| PPAR-δ |
Absent |
Extensive |
| PPAR-γ |
Absent |
Minimal |
Formalin-fixed paraffin-embedded histological sections of liver from control or
chronic ethanol fed adult rats that were treated with vehicle or a PPAR agonist,
were stained with Sirius Red to detect peri-sinusoidal fibrosis. Peri-sinusoidal Sirius
red staining was absent in control livers, irrespective of PPAR agonist treatment. In
livers from Ethanol+Vehicle and Ethanol+PPAR-δ agonist treated rats, abundant
peri-sinusoidal Sirius Red staining with a chicken-wire pattern was observed in
hepatic Zones 2 and 3. Ethanol fed rats that were treated with a PPAR-α agonist
had reduced peri-sinusoidal fibrosis relative to vehicle, and ethanol fed rats that
were treated with the PPAR-γ agonist had minimal or no evidence of peri-sinusoidal
fibrosis, similar to controls. (See Figure 4). |
