Figure 2: Characterization of the histology of Mary-X, FC-IBC01 and FC-IBC02 preclinical models of IBC that form tumor emboli. Figure 2a &2c: Light micrograph of H&E stained tumor tissue isolated from A. Mary-X, B. FC-IBC01 and C. FC-IBC02 xenografts. Insets show the presence of tumor emboli derived from the primary tumors (20 x magnifications). Figure 2d-2f. Triple color immunofluorescence and fluorescence microscopy defined the tumor emboli that express E-cadherin protein (green fluorescence) that are within the podoplanin expressing lymphatic vessels (red fluorescence) in (d). Mary-X, (e). FC-IBC01 and (f). FCIBC02 tumor xenografts. Topro-3 impacts a blue fluorescent signal to nuclear DNA.