| S. No |
Step |
Medium |
Type |
| 1 |
Fixation |
10% formal saline |
12 hours |
| 2 |
Dehydration |
Acetone |
3 changes at
intervals of 2 hours |
| 3 |
Clearing |
Benzene |
3 changes at
intervals of 2
hours |
| 4 |
Wax embedding |
Paraffin wax at 56oC |
Two changes in molten wax at its melting point were given at time intervals of 1½ hrs. & 1 hr respectively |
|
