Primer Sequence Position HBI  5′-TGCCAAGTGTTTGCTGACGC-3′ 1176–1195 HB-R 5′-AAAGTTGCATGGTGCTGGTG-3′ 1823–1804 HB2 5′-CCATACTGCGGAACTCCTAG-3′ 1266–1285 HBVF1 5′-GAAAAGCTTCCACCATGGCTGCTAGGGTGTGCTG-3′ 1374–1393 HBVR1 5′-CTAATCTCCTCCCCCAACTCCTCCCAGTC-3′ 1728–1756 HBVF2-2 5′-GTTGGGGGAGGAGATTAGGTTAATGATCTTTGTAC-3′ 1739–1773 HBVF2-3.1 5′-GTTGGGGGAGGAGAVTAGGTTAATGATCTTTGTAC-3′ 1739–1773 HBVF2-3.2 5′-GTTGGGGGAGGAGATTAGGTTAATGATTTTTGTAC-3′ 1739–1773 HBVF2-4 5′-GTTGGGGGAGGAGAVTAGGTTAATGATTTTTGTAC-3′ 1739–1773 HBVR2 5′-CATCTCGAGTTAGGCAGAGGTGAAAAAGTTGCATG-3′ 1813–1838 The underlined letters, restriction enzyme site (HindIII and XhoI); the solid letters, X gene mutant sites.

Table 1: Oligonucleotide primers used in the study.