Primer Sequence (5’→3’) Position (mRNA) Amplicon size (bp) 18S For GGA CAC GGA CAG GAT TGA CA 1278 50 Rev ACC CAC GGA ATC GAG AAA GA 1327 Cdx2 For AGA CGA GTG GGA TTG TGG AC 1190 155 Rev GAA AGC TTG GTG CCT GTA GC 1344 Tpbpa For GCC ACT GTG CCA TTG TCT AAG G 489 100 Rev GCC CCT GCT GTT TTT GCT TC 588 Prl3b1 For CAT AGT GGC AGC AGT GGC TA 360 232 Rev CAT GCA CCG ATA CAG GAC AC 591 Gjb3 For ATC TGG CTG TCG GTA GTG TTC G 67 464 Rev TAG CAA TCC ACG GTG TTG GG 530 Prl2a1 For GTG AAC AAC CTG CCA TCC TT 423 236 Rev CTA CCC TCA CAG CGG ATC AT 658 Prl5a1 For GCT GCTGCT TCT GTC AAA CTT GC 86 205 Rev CCT GTT CTG GAA TGT CTG GTG TG 290 Mmp2 For ATC CCG TTA TGA GAC CCT GAG C 145 161 Rev AAT CGT GCC TCC ATC CTT GC 305 *Cdx2: Caudal Type Homeobox 2; Tpbpa: Trophoblast Specific Protein Alpha; Prl3b1: Prolactin Family 3, Subfamily b, Member 1; Gjb3: Gap Junction Protein, Beta 3; Prl2a1: Prolactin Family 2, Subfamily a, Member 1; Prl5a1: Prolactin Family 5, Subfamily A, Member 1; Mmp2: Matrix Metalloproteinase 2; For: Forward Primer; Rev: Reverse Primer; Position: Initial Nucleotide For Primer Binding; Bp: Base Pair Size of Amplicon.

Table 2: Primer pair sequences for real-time quantitative RT-PCR*.