| Gene |
Age |
Ethanol |
Age x Ethanol |
| |
F-Ratio |
P-Value |
F-Ratio |
P-Value |
F-Ratio |
P-Value |
|
Wnt 5a |
22.79 |
< 0.0001 |
1.66 |
0.2045 |
2.36 |
0.0706 |
|
Wnt 5b |
2.128 |
0.5427 |
17.22 |
0.0116 |
16.53 |
0.0658 |
|
Fzd4 |
157.82 |
< 0.0001 |
1.59 |
0.2163 |
10.85 |
< 0.0001 |
|
Fzd6 |
2.78 |
0.0747 |
0.79 |
0.7530 |
1.97 |
0.1193 |
|
Axin2 |
54.25 |
< 0.0001 |
3.49 |
0.0390 |
9.19 |
< 0.0001 |
|
Didxc |
7.3 |
0.0021 |
1.15 |
0.3279 |
2.34 |
0.0727 |
|
EP300 |
69.87 |
< 0.0001 |
2.96 |
0.0632 |
4.46 |
0.0045 |
Quantitative duplex probe-based RT-PCR analysis was used to measure gene expression.Results were normalized to β-actin levels which remained stable with respect
to ethanol treatment and postnatal age. Results were analyzed by Two-way ANOVA with post-hoc Tukey tests for significance. F-ratios and corresponding P-values for
postnatal age, ethanol treatment and dose, and interaction between age and ethanol exposure are listed. Significant trends correspond to 0.05<P<0.10. |
