Figure 2A: CDPK genes from Arabidopsis thaliana (AK1) and potato cv. Rishiri (RiCDPK2). Diagram on the top shows AK1. The full length of RiCDPK2 from kinase-I to X with junction-domain and EF hand (KJC, 1488 bp) was expressed for the isolation of the His-tagged proteins. Kinase domains (Vib-XI) with junction domain and EF-hands (1050 bp) was expressed for the His-tagged proteins. The N-terminus, kinase domains, with junction of the RiCDPK2 (KJ) was expressed for the His-tagged proteins, and J with EF-hands (JC) was also expressed in the His-tagged proteins.