Figure 2B: His-tagged proteins from different domains of RiCDPK2, obtained from various steps of preparation were separated by electrophoresis on a SDS polyacrylamide (12%) gel and visualized with coomassie brilliant blue. Description of K-J-Ca2+, KVIb-J-Ca2+, K-J and J-Ca2+ is described in A . Lane 1, total soluble protein applied to His-bind resin affinity column; lane 2, pass through of the affinity column and lane 3, proteins eluted from affinity column. kDa indicates molecular mass markers in kDa. Arrows show the bands of the purified fusion proteins of the respective samples.