Figure 4: ELISA assay of the interaction of PiPE from P. infestans with Histagged proteins from different domains of Ca2+-dependent protein kinase (RiCDPK2) from potato cv. Rishiri (R1-gene). The His-tagged proteins were prepared from the RiCDPK2, and included kinase domain I to X with junction- and Ca2+-binding -domain (KJC), kinase-Vib to X with junction- and Ca2+-binding-domain (KVIbJC), kinase-I to X with junction-(KJ) and junctionwith Ca2+-binding-domain (JC). All the conditions were the same as those shown in Table 1. PiPE detected with PiPE-Abs served as a positive control. Experiments were repeated two times. Similar results were obtained. Each bar is the mean ± SE of two replicates.