Figure 3: sCLU level is associated with DDP sensitivity in A549 cells. (A) A549/shRNA or A549/sCLU-shRNA stable clone were seeded into 96-well plates. The following day, 1-50μM cisplatin were applied. After incubation for 72 hours, cell survival was analyzed by MTT. Cell growth in the absence of DDP corresponds to 100. (B) A549DDP was stably transfected with control or sCLU shRNA, then treated with 1-50 μM cisplatin for 72 h. MTT assays were performed the cell survival. (C) A549 cells was stably transfected with pCDNA3.1 control or pCDNA3.1-Sclu, then treated with 1-50 μM cisplatin for 72 h. MTT assays were performed the cell survival. Boxes, mean; bars, ± SD. *P<0.05, **P<0.01 (compared with control or cells transfected with mock expression plasmid).