Figure 5: Regulation of DDP-induced pERK1/2 and pAKT upregulation by sCLU. (A) A549 cells were treated with cisplatin (DDP) (1-50 μM) for 72 times. Protein expression was evaluated in whole-cell extracts from A549 cells by Western blotting. (B) A549 cells were stably transfected with sCLU-shRNA vector against sCLU, then treated with 1-50 μM cisplatin for 72 h. Protein expression was evaluated in whole-cell extracts from A549 cells by western blotting.