Figure 3: Effects of XB130 shRNA on cell growth. A: Western blot assay for XB130 in U2OS cells, XB130 shRNA-transfected U2OS cells and shRNA-transfected U2OS cells. B: Data shown (mean ± SD) here is from three independent tests. XB130 depleted U2OS cells and the control cells were seeded in 24-well plates at a density of 1×104 viable cells per well and were cultured in growth chamber for 96 hours. Then the cells were detached in trypsin-EDTA, and counted using a cell counter. Cell numbers were averaged and results expressed as percent of control.