Figure 2: ERK5 knockdown reduces cell migration and invasion. A: U2OS cells migration analysis by the wound healing assay. A scratch “wound” was made in a cell monolayer and numbers of cells having migrated were counted comparing phase contrast micrographs taken immediately and 24 h after making the wound. Shown are averages from three experiments. *P<0.01, Student's ttest. B: Matrigel invasion assay. The assay was done in modified Boyden chambers consisting of Transwell with Matrigel-coated 8- μm filter insert in 24-well plates for 24 h. Average numbers from three experiments are shown. **P<0.001, Student's t-test.