Figure 3: Expressing Slug, E-cadherin and MMP-9 in ERK5- silenced U2OS cells. A: Real-time PCR showed decreased Slug mRNA expression in U2OS cells stably expressing a control or ERK5-specific siRNA 1 and 2. B: Real-time PCR showed decreased MMP-9 mRNA expression in U2OS cells stably expressing a control or ERK5-specific siRNA 1 and 2. C: Real-time PCR showed no significant effect on E-cadherin expression in U2OS cells stably expressing a control or ERK5-specific siRNA 1 and 2. D: Western blot showed decreased Slug and MMP-9 protein expression in U2OS cells stably expressing a control or ERK5-specific siRNA 1 and 2. Restoration of Slug expression in ERK5-silenced U2OS cells increased the MMP-9 protein levels but not the E-cadherin levels. β-Actin was a loading control. (Vs control,*P<0.05).