Figure 4: Re-expressing Slug and MMP-9 in ERK5-silenced U2OS cells increases cell migration and invasion. A: In vitro wound healing assay showing the effect of Slug and MMP-9 overexpression on migration of ERK5 siRNA1/2 cells. B: In vitro Matrigel assay showing the effect of Slug and MMP-9 overexpression on invasiveness of ERK5 siRNA1/2 cells. Vs Slug or MMP-9 groups, *P<0.05, Student's t-test.