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Figure 1: Frequency of successful amplification in 26 samples with 5 groups of primer sets. By confirming the positive amplicon on electrophoresis, the amplification ability of five groups of primer sets (A, B, C, D, and E) was evaluated. The group D primer set had the highest frequency of successful amplification for KIT exon 9 and the group A primer set had the highest frequency of successful amplification for the other exons. |