| Antibody to |
Manufacturer |
Working Dilution |
Antigen retrieval |
Buffer pH |
Incubation time and temperature |
Secondary antibody |
| CD117 |
DAKO A4502 |
0.180555556 |
MW/40min |
6 |
1h, RT |
En Vision |
| CD34 |
Nichirei413111 |
RU |
None |
- |
1h, RT |
En Vision |
| PDGFR-α |
Santa Cruz sc-338 |
0.388888889 |
MW/20min |
6 |
1h, RT |
ABC |
| DOG1 |
Bio SB BSB6723 |
1:50 |
MW/40min |
8 |
1h, RT |
ABC |
| S-100 |
DAKO Z0628 |
2.819444444 |
None |
- |
24h, 4C |
En Vision |
| α-SMA |
DAKO M0851 |
0.319444444 |
None |
- |
24h, 4C |
En Vision |
| Desmin |
DAKO M0760 |
0.111111111 |
MW/40min |
8 |
1h, RT |
En Vision |
| Ki-67 |
DAKO M7240 |
0.597222222 |
AC121°C/20min |
6 |
24h, 4C |
En Vision |
ABC: ABC immunoperoxidase kit (Vector Laboratories); AC: Autoclave; En Vision: En Vision + System (DAKO A/S); MW: Microwave; RT: Room Temperature; RU: Ready
to Use |
