| Exon |
group A primers* |
group B primers* |
group C primers* |
group D primers* |
group E primers* |
| Exon 9 |
F:TCCTAGAGTAAGCCAGGGCTTTTG |
F:TTCCTAGAGTAAGCCAGGGC |
F:GCCACATCCCAAGTGTTTTATG |
F:ATGCTCTGCTTCTGTACTGCC |
F:GTATGCCACATCCCAAGTGT |
| R:TGGTAGACAGAGCCTAAACATCCC |
R:CCTAAACATCCCCTTAAATTGG |
R:GAGCCTAAACATCCCCTTAAATTG |
R:CAGAGCCTAAACATCCCCTTA |
R:CATGACTGATATGGTAGACA |
| Exon 9A (284bp) |
Exon 9B (273bp) |
Exon 9C (310bp) |
Exon 9D (238bp) |
Exon 9E (334bp) |
| Exon 11 |
F:CTCTCTCCAGAGTGCTCTAATGAC |
F:TGTTCTCTCTCCAGAGTGCTCTAA |
F:CCAGAGTGCTCTAATGACTG |
F:CCTTTGCTGATTGGTTTCGT |
F:CCAGAGTGCTCTAATGACTG |
| R:GGTGACATGGAAAGCCCCTGTTTC |
R:ACCCAAAAAGGTGACATGGA |
R:AGCCCCTGTTTCATACTGAC |
R:AAACAAAGGAAGCCACTGGA |
R:GGAAGCCACTGGAGTTCCTT |
| Exon 11A (233bp) |
Exon 11B (246bp) |
Exon 11C (223bp) |
Exon 11D (382bp) |
Exon 11E (274bp) |
| Exon 13 |
F:TGCGCTTGACATCAGTTGCCAG |
F:TGCCAGTTGTGCTTTTTGCTA |
F:CTTGACATCAGTTTGCCAGTTGT |
F:CATCAGTTTGCCAGTTGTGC |
F:GACATCAGTTTGCCAGTTGT |
| R:AAGGCAGCTTGGACACGGCTTTAC |
R:GCTTTACCTCCAATGGTGCAG |
R:GACAGACAATAAAAGGCAGCTTG |
R:ACACGGCTTTACCTCCAATG |
R:TGTTTTGATAACCTGACAGAC |
| Exon 13A (196bp) |
Exon 13B (161bp) |
Exon 13C (203bp) |
Exon 13D (173bp) |
Exon 13E (214bp) |
| Exon 17 |
F:CTCCTCCAACCTAATAGTGTATTCAC |
F:ATGGTTTTCTTTTCTCCTCC |
F:TGGTTTTCTTTTCTCCTCCAA |
F:TGTATTCACAGAGACTTGGC |
F:GCAACACTATAGTATTAAAAAG |
| R:TGTCAAGCAGAGAATGGGTACTCAC |
R:TACATTATGAAAGTCACAGG |
R:GCAGGACTGTCAAGCAGAGA |
R:GGATTTACATTATGAAAGTCACAGG |
R:CCTTTGCAGGACTGTCAAGCA |
| Exon 17A (165bp) |
Exon 17B (243bp) |
Exon 17C (184bp) |
Exon 17D (218bp) |
Exon 17E (248bp) |
| Exon 12 |
F:CTCTGGTGCACTGGGACTTTGGT |
F:CTCTGGTGCACTGGGACTTT |
F:CTCTGGTGCACTGGGACTTT |
F:TCTGGTGCACTGGGACTTTG |
F:TCCAGTCACTGTGCTGCTTC |
| R:CTTGGGAGGTTACCCCATGGAAC |
R:GGAGGTTACCCCATGGAACT |
R:GCAAGGGAAAAGGGAGTCTT |
R:AGCTCAGATCTCTATTCTGC |
R:GCAAGGGAAAAGGGAGTCTT |
| Exon 12A (216bp) |
Exon 12B (212bp) |
Exon 12C (233bp) |
Exon 12D (280bp) |
Exon 12E (272bp) |
| Exon 18 |
F:CACCATGGATCAGCCAGTCTTGC |
F:CATTTCTTCCTTTTCCATGCA |
F: CTTGCAGGGGTGATGCTATT |
F:GATCAGCCAGTCTTGCAGGG |
F:ACCATGGATCAGCCAGTCTT |
| R:TGAAGGAGGATGAGCCTGACCAG |
R:TGTGGGAAGTGTGGACGTAC |
R:AGAAGCAACACCTGACTTTAGAGATTA |
R:TGCCAAGGCAGTGTACTGAC |
R:TGAAGGAGGATGAGCCTGACC |
| Exon 18A (252bp) |
Exon 18B (165bp) |
Exon 18C (230bp) |
Exon 18D (341bp) |
Exon 18E (251bp) |
| PCR Conditions |
For exon 11A |
For all the exons B |
For all the exons C |
For all the exons D |
For exons 9E and 13E |
| 94°C-30s-52°C-30s-72°C-30s/35Cy |
94°C-30s-55°C-20s-65°C-20s/40Cy |
95°C-30s-60°C-30s-72°C-1m/30Cy |
95°C-30s-60°C-30s-72°C-30s/34Cy |
94°C-1m-54°C-1m-72°C-2m/40Cy |
| For exon 9A, 13A, 17A, 12A and 18A |
|
|
|
For exon 17E |
| 94°C-30s-61°C-30s-72°C-30s/35Cy |
|
|
|
94°C-1m-50°C-1m-72°C-2m/40Cy |
| |
|
|
|
For exons 11E, 12E and 18E |
| |
|
|
|
94°C-1m-55°C-1m-72°C-2m/40Cy |
Cy: Cycle; m: Minute; s: Second. *Group A primer sets were designed in our laboratory. Group B, C, D, and E primer sets were used in references 5, 6, 7, and 8, respectively.