| Primer Name |
Primer sequence |
Annealing temperature |
Product Size (bp) |
| Exon 9 c-KIT |
Forward5’- ATG CTC TGC TTC TGT ACT GCC-3’
Reverse 5’- CAG AGC CTA AAC ATC CCC TTA –3’ |
58°C |
238 bp |
| Exon 11 c-KIT |
Forward 5’- GAT CTA TTT TTC CCT TTC TC-3’
Reverse 5’- AGC CCC TGT TTC ATA CTG AC –3’ |
57°C |
174 bp |
| Exon 13 c-KIT |
Forward 5’- GCT TGA CAT CAG TTT GCC AG-3’
Reverse 5’- AAA GGC AGC TTG GAC ACG GCT TTA –3’ |
66°C |
193 bp |
| Exon 14 c-KIT |
Forward 5’- GTC TGA TCC ACT GAA GCT G-3’
Reverse 5’- ACC CCA TGA ACT GCC TGT C –3’ |
59°C |
151 bp |
| Exon 17 c-KIT |
Forward 5’- TAC AAG TTA AAA TGA ATT TAA ATG GT-3’
Reverse 5’- AAG TTG AAA CTA AAA ATC CTT TGC –3’ |
63°C |
228 bp |
| Exon 12 PDGFRa |
Forward 5’-TCC AGT CAC TGT GCT GCT TC-3’
Reverse 5’-GCA AGG GAA AAG GGA GTC TT-3’ |
56°C |
260 bp |
| Exon 14 PDGFRa |
Forward 5’-GTA GCT CAG CTG GAC TGA TA-3’
Reverse 5’-AAT CCT CAC TCC AGG TCA GT-3’ |
55°C |
180 bp |
| Exon 18 PDGFRa |
Forward 5’-TAC AGA TGG CTT GAT CCT GAG T-3’
Reverse 5’-AGT GTG GGA GGA TGA GCC TG-3’ |
59°C |
211 bp |
| Exon 12 PDGFRB |
Forward 5’-TAA TTC CTG GGG TTG GTC CTC-3’
Reverse 5’-AAC TTG AGT CCC CAC ACT GCC-3’ |
52°C |
174 bp |
| Exon 14 PDGFRB |
Forward 5’-GGG GCA GAA GAG TCA GAA TAG-3’
Reverse 5’-GGA GTG TGC TGT TGT GCA AG-3’ |
63°C |
300 bp |
| Exon 18 PDGFRB |
Forward 5’-CCC AAA GCC CTT GAC ATG AAG-3’
Reverse 5’-ACT GGT CAG GAG GGA ATC TG-3’ |
56°C |
274 bp |
