Figure 3: Various durations of staining (A 16 hours, B 8 hours, C 4 hours), while maintaining a temperature of 4°C and a concentration of 2.5 μl CMFDA (25 μmol) to 5 μl EthD-1 (10 μmol) to 1 ml DMEM. (40x objective. Note that the 16-hour sample was cut longitudinally rather than transversely).