Figure 1: Polyamine in culture medium Lactobacillus sakei No.2 by HPLC. Three polyamines (putrescine, spermidine, and spermine) were determined by comparing with standard polyamines. All polyamines (putrescine, spermidine, and spermine) were detected in culture medium, and the detected polyamines were collected by HPLC with a fraction collector.