Figure 2: This graphically illustrates the relative expression of mast cell protease-4 (MCPT-4) by RBL 2H3 cells following treatment for seven days with the indicated ethanol concentration. Cells were treated continually with the respective ethanol concentration. Medium was changed daily during the treatment period. MCPT-4 expression was assayed by semiquantitative polymerase chain reaction. Expression of MCPT-4 was normalized to acidic ribosomal binding protein and the experimental treatments are expressed relative to the no ethanol control. Significance was determined by one way Anova comparison to the no ethanol control (*<0.05; n=3 independent cultures).