Figure 2: Flow cytometry of CD3+, CD4+, CD8+ T-cells in normal and infected spleen of BALB/c mice. Isolated MNCs of normal and P. berghei (NK-65) infected spleen were stained with antibody viz. Anti-mouse CD3 (FITC conjugated), CD4 (PE conjugated) and CD8 (APC conjugated). Significant variation in the frequencies of CD3+, CD4+ , CD8+ T cells was observed with rise in parasitemia, Data expressed are the mean ± S.D. of the percentage of CD3+, CD4+, CD8+ T cells (A-C-Normal spleen; D-R- P. berghei infected spleen).