Figure 3: Flow cytometry of various T cell populations in spleen of GP-IV. Isolated MNCs of immunized spleen were stained with antibody viz. Anti-mouse CD3 (FITC conjugated), CD4 (PE conjugated) and CD8 (APC conjugated). Estimation of total lymphocytes and subset analysis was performed on various days of experiment. With TPA immunization significant increase (p<0.005) was observed in CD3+, CD4+, CD8+ T cells. Data expressed are the mean ± S.D. of the percentage of various cells.