Figure 4: Flow cytometry of CD4+Tregs in normal, infected and immunized BALB/c mice spleen. Isolated MNCs of normal, P. berghei (NK-65) infected and immunized spleen were stained with antibody viz. Anti CD4 (FITC conjugated), Anti CD25 (APC conjugated), Anti FoxP3 (PE conjugated). Estimation of CD4+ Tregs analysis was performed on various days (D0, D1, D3, D5 and D7) of experiment. Data expressed are the mean ± S.D. of the percentage of CD4+ Treg cells (A-Normal spleen; B and C-Infected spleen; D and E-Placebo control; G and I-Immunized spleen; FAfter parasite clearance).