Figure 5: Antibody titers observed in sera of mice immunized with TPA of P. berghei by IFA assay. BALB/c mice were immunized with TPA of P. berghei emulsified in FCA on day 0 and FIA on D14 and D28. Animals immunized with PBS and adjuvant alone was termed as placebo control. Animals were challenged with 1 × 106 parasitized erythrocytes on day 7-post final immunization. Serum obtained was serially diluted in two fold dilutions and end-point titers were determined as the highest serum dilutions giving detectable fluorescence.