Figure 7: Level of various cytokines in the sera of TPA immunized BALB/c mice. Animals were immunized with TPA emulsified FCA/ FIA along with placebo control animals on similar days. Sera samples were collected from animals of various groups on D7 post final immunization before challenge and were analyzed for the presence of IL-2, IL-4, IL-10 and IFN-γ by sandwich ELISA. Data expressed are the mean ± S.D. of the cytokine concentrations from two different assays. *p<0.001; **p<0.0001.