Figure 1: FUS1 mRNA and protein levels. (A) FUS1 mRNA levels in the indicated cell lines as evaluated by semi-quantitative RT-PCR. GAPDH was included as control for mRNA integrity and cDNA synthesis. (B) Western blot analysis of the level of endogenous FUS1 in the SCLC cell lines, GLC16, H69, DMS273 and DMS53. H1299 was included as a negative control and GLC16 cells transiently transfected with wt-FUS1 expression vector (GLC16-FUS1) was included as positive control. GAPDH was used as control for protein loading.